It might be cleaner to use an IRES sequence right after the stop codon of SYCP3 so your fluorescent reporter expresses off the same transcript without being conjugated directly to SYCP3. Would be an issue if you were imaging for SYCP3 localization, but you just want flow sorting, right?
It might be cleaner to use an IRES sequence right after the stop codon of SYCP3 so your fluorescent reporter expresses off the same transcript without being conjugated directly to SYCP3. Would be an issue if you were imaging for SYCP3 localization, but you just want flow sorting, right?
It's a pretty well-known technology and I don't know the literature very well for it, but a bit of searching was able to find me this: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2275123/