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Mar 26Liked by Metacelsus

this was my responses from GTP-4 (I indeed got primordial germ cells for #1)

These are marker genes for different types of human cells. Here are the cell types that these marker genes are associated with:

SOX17, POU5F1, NANOS3, PRDM1, NANOG, CD38 - Primordial Germ Cells

POU5F1, SOX2, KLF4, ITGA6, NANOG - Induced Pluripotent Stem Cells

SOX17, FOXA2, CXCR4, GATA4 - Definitive Endoderm Cells

FOXL2, AMHR2, CD82, NR5A1, FSHR, GATA4 - Granulosa Cells

ZP3, DPPA3, DDX4, NPM2, ZP2 - Oocytes

FOXL2, FSHB, NR5A1, PITX1, GNRHR - Pituitary Gonadotrope Cells

STK31, ZBTB16, DDX4, SSEA4, NANOS2 - Spermatogonial Stem Cells

NR2F2, CYP17A1, STAR, LHCGR, GLI1, HSD3B - Leydig Cells

CASC3, PGAP1, SLC6A16, CNTNAP4, NPHP1 - These genes are not specific to a single cell type and are expressed in various tissues.

SYCP1, TEX12, REC8, SPO11, SYCP3 - Meiotic Cells

NR5A1, SOX9, FSHR, GATA4 - Sertoli Cells

OTX2, SOX1, TUBB3, PAX6 - Neural Progenitor Cells

Please note that these are general associations and the presence of these genes does not definitively determine the cell type. The exact cell type can depend on the expression levels of these genes and the presence of other marker genes.

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It's interesting that it didn't say "unknown" for #9, but used a different phrase to represent uncertainty.

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Mar 25Liked by Metacelsus

Here are GPT-4's responses:

Prompt 1:

Pluripotent Stem Cells

Embryonic Stem Cells

Endoderm Cells

Granulosa Cells

Oocytes

Gonadotroph Cells

Spermatogonial Stem Cells

Leydig Cells

Neuronal Cells

Meiotic Cells

Sertoli Cells

Neural Progenitor Cells

Prompt 2:

Pluripotent Stem Cells

Embryonic Stem Cells

Endoderm Cells

Granulosa Cells

Oocytes

Pituitary Gonadotrope Cells

Germ Cells

Leydig Cells

unknown

Meiotic Cells

Sertoli Cells

Neural Progenitor Cells

Prompt 3:

Based on the marker genes provided for each row and without conducting a detailed gene expression analysis or referencing a specific database, here's an informed guess for the cell types based on common knowledge in cell biology and gene function:

CASC3, PGAP1, SLC6A16, CNTNAP4, NPHP1 - Given these genes, this profile does not clearly point to a specific, well-known cell type without further context. CNTNAP4 is involved in neuronal activity, but the combination of genes does not provide a clear match. unknown

IL9, SLC30A4, SX18P8, CHRDL2, SMUG1P1 - IL9 is a cytokine involved in immune responses, suggesting these cells might be related to the immune system. However, without a clear connection between all the markers, pinpointing a specific immune cell type is challenging. unknown

HCST, EXOSC8, ORC3, CIDECP2, DNM2 - HCST is involved in the activation of natural killer (NK) cells, suggesting this could be a profile related to NK cells or another cell type involved in the immune response. Natural Killer Cell

DTL, U3, DDX28, WDFY3, PPP1R2P4 - This combination of genes does not correspond to a specific cell type that can be easily identified without further information. These genes are involved in various cellular processes, including DNA repair and synthesis, making it hard to narrow down. unknown

LTK, STK32C, SMIM9, DPPA3P10, MTCO1P12 - LTK is a receptor tyrosine kinase that has been implicated in neuronal development and other processes, but the combination of genes provided does not specify a particular cell type without additional context. unknown

These guesses are made with caution, as identifying cell types solely based on a small set of marker genes without additional context (such as expression levels, cell function, or tissue of origin) can be quite speculative and may not accurately represent the cell types.

Prompt 3 without unknown sentence:

Identifying cell types based on marker gene expression is a nuanced task that requires a deep understanding of gene expression patterns across different tissues and developmental stages. However, I'll provide a general identification of cell types based on the marker genes listed, keeping in mind that the expression of these genes can vary under different conditions and might not be exclusive to a single cell type. Here are potential cell types based on the listed marker genes:

CASC3, PGAP1, SLC6A16, CNTNAP4, NPHP1 - These genes are involved in various cellular functions, including cell adhesion, signaling, and metabolism. Neurons or neural progenitor cells might express these, particularly due to CNTNAP4's involvement in the nervous system.

IL9, SLC30A4, SX18P8, CHRDL2, SMUG1P1 - IL9 is a cytokine that plays roles in various aspects of the immune response. This set of genes could be indicative of T helper cells, specifically Th9 cells, given IL9's role in these cells.

HCST, EXOSC8, ORC3, CIDECP2, DNM2 - HCST is involved in the immune system's activation and signaling pathways. This gene set might suggest Natural Killer (NK) cells due to HCST's significance in NK cell-mediated cytotoxicity.

DTL, U3, DDX28, WDFY3, PPP1R2P4 - These genes are involved in DNA replication, repair, and cell cycle regulation. They could be indicative of cells with high proliferative capacity, such as stem cells or progenitor cells in rapidly renewing tissues.

LTK, STK32C, SMIM9, DPPA3P10, MTCO1P12 - LTK is a receptor tyrosine kinase involved in various cell signaling pathways. This set of genes could suggest endothelial cells due to their roles in vascular development and maintenance.

It's important to note that this identification is very general and should be validated with more specific functional assays or gene expression analyses in the context of the tissue of interest.

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Thanks! These look better, although GPT-4 still can't recognize primordial germ cells.

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Mar 25Liked by Metacelsus

Claude 3 Opus:

1. Primordial germ cell

2. Pluripotent stem cell

3. Endoderm cell

4. Granulosa cell

5. Oocyte

6. Gonadotroph

7. Spermatogonial stem cell

8. Leydig cell

9. Unknown

10. Spermatocyte

11. Sertoli cell

12. Neural progenitor cell

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author

Apparently Claude 3 *can* recognize primordial germ cells, bravo! And it wasn't thrown off by the random genes. Theca cells and Leydig cells are very similar, so #8 can get partial credit.

11.5/12 by my count.

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https://copilot.microsoft.com/ Seems to work even better (using prompt 2)

Here are the cell types corresponding to each row of marker genes:

Primordial Germ Cells

Induced Pluripotent Stem Cells

Definitive Endoderm Cells

Granulosa Cells

Oocytes

Pituitary Gonadotrope Cells

Spermatogonial Stem Cells

Theca Cells

Unknown

Meiotic Cells

Sertoli Cells

Neural Progenitor Cells

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